Conference Proceedings
The effect of TrkB receptor knockdown on mouse retinal ganglion cell function and their response to acute mild intraocular pressure stress
Vickie Hoi Ying Wong, Amy Wang, Christine Tram Oanh Nguyen, Jeremiah Kah Heng Lim, Madeline Nicholson, Junhua Xiao, Simon Murray, Bang V Bui
Investigative Ophthalmology and Visual Science | Association for Research in Vision and Ophthalmology | Published : 2019
Abstract
Purpose : To examine the effect of tropomyosin receptor kinase B (TrkB) receptor removal on basal retinal ganglion cell (RGC) function and recovery from acute intraocular pressure (IOP) stress following conditional deletion of neuronal-specific TrkB receptors in adult mice. Methods : Conditional TrkB receptor knockout (KO) from Thy-1 positive neurons was induced via daily tamoxifen injections (100 ul i.p. 75mg/kg, 5 days) in 3-month old Thy1-GFP CreERT2+/- TrkBfl/fl transgenic (TrkB KO n=12) and control CreERT2-/- TrkBfl/fl mice (n=12). Four weeks later, one eye was exposed to controlled IOP stress of 50mmHg for 30 minutes, achieved via anterior chamber cannulation (50 μm glass micropipette..
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